Whole genome analysis: Experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR

Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR.

The recent ability to sequence whole genomes allows ready access to all genetic material. The approaches outlined here allow automated analysis of sequence for the synthesis of optimal primers in an automated multiplex oligonucleotide synthesizer (AMOS). The efficiency is such that all ORFs for an organism can be amplified by PCR. The resulting amplicons can be used directly in the construction...

Whole-Genome Amplification by Degenerate Oligonucleotide Primed PCR (DOP-PCR).

INTRODUCTIONPCR-based whole-genome amplification (WGA) has the goal of generating microgram quantities of genome-representative DNA from picogram or nanogram amounts of starting material. This amplification should introduce little, or ideally no, representational bias. Unlike other techniques for WGA, PCR-based methods are generally less affected by DNA quality and are more applicable to DNA ex...

Whole Genome Sequencing: An Efficient Approach in Food Safety Management System

OligoArray: genome-scale oligonucleotide design for microarrays

SUMMARY OligoArray is a program that computes gene specific and secondary structure free oligonucleotides for genome-scale oligonucleotide microarray construction or other applications. AVAILABILITY The program code is distributed under the GNU General Public License and is freely available for non-profit use via request from the authors.

Won For All: How the Drosophila Genome Was Sequenced

In The Strongest Boy’s title essay, Reilly tells the story of a German newborn whose strangely muscled physique is due to a frame shift mutation in the aforementioned muscle inhibitor, myostatin. As a result, his 7-month-old thighs look like those of a miniscule Lance Armstrong. His mother, a heterozygote and professional athlete, already has hidden him from public view, shielding him from scru...